Logics of Transposon sequencing

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Tags: pipeline

  1. original reads:
  2. >read_1/1
  3.     AAGAGTTAGTAGGGCGGGCGATTAAGGGCAAACGTGATCAGGTCTTTTTAGCGACTAAGT
  4. TCGGTATTGTGCGTGACCCCACTGATCCCAGGGTACGCGGTGTGAGCAGCCGTCCTGATT
  5. ATATCCGCCAGTCG  GTTGATGAGAGCTTTGTTGTAGGTG
  7. TODO_TOMORROW: follow the logics below!
  8. vs. NEB_i5_Tn_02
  9. 5'-ACACTCTTTCCCTACACGACGCTCTTCCGATCTCACCTACAACAAAGCTCTCATCAAC-3'-bacterial_genomics
  10. duplicate_PCR generate rc fragments
  11.                  adapter_removed!  |GTGGA  ...     AGAGTAGTTG                       ...  GATTGAGAA
  12.                                                                                  <-------read_R1--
  14. >read_1/2
  15. AATGAAGAGTTAGTAGGGCGGGCGATTAAGGGCAAACGTGATCAGGTCTTTTTAGCGACT
  16. AAGTTCGGTATTGTGCGTGACCCCACTGATCCCAGGGTACGCGGTGTGAGCAGCCGTCCT
  17. GATTATATCCGCCAGTCGGTTGATG  AGAGCTTTGTTGT

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