Author: gene_x

Abstract: Using spaceranger to process spatial transcriptomics data involves several steps, from preparing the necessary input files to running the analysis and interpreting the results. Below, I'll provide a c

Author: gene_x

Abstract: 1. prepare input reads and samplesheet ln -s ./F23A430001245_SEPiovzP/soapnuke/clean/EX_15_min_A/EX_15_min_A_1.fq.gz . ln -s ./F23A430001245_SEPiovzP/soapnuke/clean/EX_15_min_A/EX_15_

Author: gene_x

Abstract: 1. Restricting User 'malawi' from Installing System-wide Programs and Verifying Permissions chmod o-rx /home/jhuang ls -ld /home/jhuang cd /home/jhuang groups malawi

Author: gene_x

Abstract: 1. nextflow processing data (chipseq) jhuang@hamm:/mnt/h1/jhuang/DATA/Data_Denise_LT_DNA_Binding/Histone-ChIP_hg38/H3K27ac_H3K4me1_public$ nextflow run NGI-ChIPseq/main.nf --reads '/mnt/h1/jh

Author: gene_x

Abstract: Cell free circulating DNA (cfDNA) refers to DNA fragments present outside of cells in body fluids such as plasma, urine, and cerebrospinal fluid (CSF). CfDNA was first identified in 1948 from plasma o

Author: gene_x

Abstract: [](/static/sT_revision/PCA_3D.png "PCA_3D") [](/static/sT_revi

Author: gene_x

Abstract: kraken2 --db Minikraken2_v2 input_sequences.fasta --output output.kraken2 kraken2 --db=/home/jhuang/Tools/k2_standard_20230605 --output=A10_output.txt --report=A10_report.txt --paired ./results_ATCC1

Author: gene_x

Abstract: 1. input data #Deciphering Evolutionary Trajectories in Acinetobacter baumannii: A Comparative Study of Isolates ATCC 19606, AYE, and ATCC 17978 #PRJNA1041744

Author: gene_x

Abstract: 1. download viral database cp download_db.py down_db.py.backup sed -i -e 's/39733/1396/g' download_db.py #NOTE the download_db.py cannot run alone, rather than via "vrap.py -u

Author: gene_x

Abstract: 摘要 自现代转座子插入测序（TIS）方法引入以来已有10年的时间了。这些方法结合了全基因组转座子突变和高通量测序，用于估计细菌基因组中每个遗传组分的适应性贡献或必需性。2009年，发布了四种TIS变体：转座子测序（Tn-Seq）、转座子定向插入位点测序（TraDIS）、插入测序（INSeq）和高通量插入跟踪深度测序（HITS）。此后，TIS已成为分子微生物学家的重要工具之一，它是少数直接将表