gene_x 0 like s 16 view s
Tags: packages, python, pipeline
https://lumicks-pylake.readthedocs.io/en/v0.8.2/install.html#updating
https://github.com/JamesLiWan/MultimerizationCode/blob/main/C_Trap-Multimer-photontrack.ipynb
Fix which pylake we should use for the legacy-code.
Yep – you’re absolutely right, the channel parameter was added in pylake v0.9.0. If you look at the changelog:
v0.9.0 (Jul 29 2021) introduces unit support and other updates, which also aligns with introducing the channel argument in track_greedy()
Versions before v0.9.0, like v0.8.2 (Apr 30 2021), use the old signature:
track_greedy(kymograph, line_width, pixel_threshold, …)
with no channel argument.
Since your code uses track_greedy(data, …) without specifying channel, you should install: pylake v0.8.2
Create mamba environment for pylake v0.8.2
mamba env remove -n pylake_082_clean
mamba env create -f pylake_082_env.yml
name: pylake_082_clean
channels:
- conda-forge
- defaults
dependencies:
- python=3.8
- pip
- jupyterlab
- ipywidgets
- numpy
- matplotlib
- h5py
- pandas
- scipy
mamba activate pylake_082_clean
#IMPORTANT:
mamba install -c conda-forge lumicks.pylake=0.8.2
mamba install -c conda-forge numpy matplotlib ipywidgets scipy scikit-image pandas
python -c "import lumicks.pylake; print(lumicks.pylake.__version__)"
mamba install notebook=6
#+ ipython_genutils 0.2.0 pyhd8ed1ab_1 conda-forge Cached
#+ nbclassic 1.1.0 pyhd8ed1ab_0 conda-forge Cached
#+ notebook 6.5.7 pyha770c72_0 conda-forge Cached
#Downgrade:
#─────────────────────────────────────────────────────────────────────
#- jupyter_client 8.6.3 pyhd8ed1ab_0 conda-forge Cached
#+ jupyter_client 7.4.9 pyhd8ed1ab_0 conda-forge Cached
jupyter notebook --version
#pip show notebook
(pylake_082_clean) jhuang@WS-2290C:/mnt/md1/DATA/Data_Vero_Kymographs$ mamba list jupyter
# packages in environment at /home/jhuang/mambaforge/envs/pylake_082_clean:
#
# Name Version Build Channel
jupyter-lsp 2.2.5 pyhd8ed1ab_0 conda-forge
jupyter_client 7.4.9 pyhd8ed1ab_0 conda-forge
jupyter_core 5.8.1 pyh31011fe_0 conda-forge
jupyter_events 0.10.0 pyhd8ed1ab_0 conda-forge
jupyter_server 2.14.2 pyhd8ed1ab_0 conda-forge
jupyter_server_terminals 0.5.3 pyhd8ed1ab_0 conda-forge
jupyterlab 4.3.0 pyhd8ed1ab_0 conda-forge
jupyterlab_pygments 0.3.0 pyhd8ed1ab_1 conda-forge
jupyterlab_server 2.27.3 pyhd8ed1ab_0 conda-forge
jupyterlab_widgets 3.0.13 pyhd8ed1ab_0 conda-forge
(pylake_082_clean) jhuang@WS-2290C:/mnt/md1/DATA/Data_Vero_Kymographs$ mamba list notebook
# packages in environment at /home/jhuang/mambaforge/envs/pylake_082_clean:
#
# Name Version Build Channel
notebook 6.5.7 pyha770c72_0 conda-forge
notebook-shim 0.2.4 pyhd8ed1ab_0 conda-forge
#Note that Notebook v6.x and Notebook v7 is different, we have to notebook v6 More Detailed Breakdown:
#Version Backend Used Notes
#Notebook v6.x and below notebook (classic Tornado-based server) No jupyter_server dependency
#Notebook v7+ Uses jupyter_server under the hood Required dependency
Install extension for the recognization of %%javascript, %matplotlib inline, and %matplotlib notebook
mamba install widgetsnbextension -c conda-forge
jupyter nbextension enable --py widgetsnbextension --sys-prefix
#ModuleNotFoundError: No module named 'ipympl', but we don't need it! jupyter nbextension enable --py ipympl --sys-prefix
jupyter nbextension list
BUG_1: ModuleNotFoundError: No module named 'matplotlib_venn'
mamba install -c conda-forge matplotlib-venn
BUG_2: TypeError: init() got an unexpected keyword argument 'drawtype'
#indicates that your version of Matplotlib is too new, and it no longer supports the drawtype argument in matplotlib.widgets.RectangleSelector. Only <= 3.4.3 supprts drawtype!
mamba list matplotlib
# packages in environment at /home/jhuang/mambaforge/envs/pylake_082_clean:
#
# Name Version Build Channel
#matplotlib 3.7.3 py38h578d9bd_0 conda-forge
#matplotlib-base 3.7.3 py38h58ed7fa_0 conda-forge
#matplotlib-inline 0.1.7 pyhd8ed1ab_0 conda-forge
#matplotlib-venn 1.1.1 pyhd8ed1ab_0 conda-forge
#DEBUG: # MODIFIED: Delete "drawtype='box'," in kymowidget.py
self.area_selector = RectangleSelector(self._axes, self.track_kymo,
useblit=True,
button=[3],
minspanx=5, minspany=5,
spancoords='pixels',
interactive=False)
OPTIONAL_BUG (NOT_DONE): [W 13:13:49.115 NotebookApp] Error loading server extension jupyterlab
#Traceback (most recent call last):
# File "/home/jhuang/mambaforge/envs/pylake_082/lib/python3.8/site-packages/notebook/notebookapp.py", line 2050, in init_server_extensions
# func(self)
# File "/home/jhuang/mambaforge/envs/pylake_082/lib/python3.8/site-packages/jupyterlab/serverextension.py", line 71, in load_jupyter_server_extension
# extension.initialize()
# File "/home/jhuang/mambaforge/envs/pylake_082/lib/python3.8/site-packages/jupyterlab/labapp.py", line 921, in initialize
# super().initialize()
# File "/home/jhuang/mambaforge/envs/pylake_082/lib/python3.8/site-packages/jupyter_server/extension/application.py", line 437, in initialize
# self._prepare_handlers()
# File "/home/jhuang/mambaforge/envs/pylake_082/lib/python3.8/site-packages/jupyter_server/extension/application.py", line 327, in _prepare_handlers
# self.initialize_handlers()
# File "/home/jhuang/mambaforge/envs/pylake_082/lib/python3.8/site-packages/jupyterlab/labapp.py", line 735, in initialize_handlers
# page_config["token"] = self.serverapp.identity_provider.token
#AttributeError: 'NotebookApp' object has no attribute 'identity_provider'
jupyter serverextension disable jupyterlab
mamba remove jupyterlab (or pip uninstall jupyterlab)
Run notebook on (pylake_082_clean) jhuang@WS-2290C:/mnt/md1/DATA/Data_Vero_Kymographs/MultimerizationCode-main_v082
(pylake_082_clean) jhuang@WS-2290C:/mnt/md1/DATA/Data_Vero_Kymographs/MultimerizationCode-main_v082$ jupyter notebook
#jupyter notebook --no-browser --port=8888
点赞本文的读者
还没有人对此文章表态
没有评论
MicrobiotaProcess Group2 vs Group6 (v1)
Small RNA sequencing processing in the example of smallRNA_7
© 2023 XGenes.com Impressum