Author: huang

Abstract: - https://www.ebi.ac.uk/sites/ebi.ac.uk/files/content.ebi.ac.uk/materials/2014/140217_AgriOmics/dan_bolser_snp_calling.pdf Common artifacts in NGS alignments that gave rise to a false-positive de nov

Author: huang

Abstract: For WGS, total DNA of K. pneumoniae isolates DT1 and DT12 was extracted on a QiaSymphony SP instrument using QiaSymphony mericon extraction kits (Qiagen). WGS libraries were constructed with NEB NextU

Author: huang

Abstract: export PERL5LIB=$CONDA_PREFIX/lib/perl5/site_perl/5.22.0/ roary -p 4 -f ./roary -i 95 -cd 99 -s -e -n -v prokka/BK036327/BK036327.gff prokka/SX003539/SX003539.gff prokka/SX010463/SX010463.gff prokka/

Author: huang

Abstract: Total RNA of triplet replicates of five conditions (control, TFEB-WT 3h dox + 21h chase, TFEB-WT 24h chase, TFEB-K3R 3h dox + 21h chase, TFEB-K3R 24h chase) was harvested using XXXX extraction kit acc

Author: huang

Abstract: https://www.ncbi.nlm.nih.gov National Center for Biotechnology Information, National Library of Medicine, NationalInstitutes of Health https://www.cncb.ac.cn/ China National Center for Bioinf

Author: huang

Abstract: ```sh ---------- updated ------------------------ Step 3: replace the readgroup in the bams S1/unique/S1.realigned.bam S2/unique/S2.realigned.bam S3/unique/S3.realigned.bam S4/unique/S4.realigned