There are 326 articles for you to read.

Creating an hg38 Database File for DiffReps Analysis

Author: gene_x

Abstract: To generate a database file for the hg38 human genome assembly for use with the DiffReps tool, you will first need to download the hg38 annotation file in GTF or GFF format, and then convert it to the

H3K4me3, H3K27me3 and TF

Author: gene_x

Abstract: H3K4me3(trimethylated histone H3 lysine 4)和H3K27me3(trimethylated histone H3 lysine 27)是组蛋白修饰的一种,与基因表达调控密切相关。转录因子(transcription factor)则是一类能够调控基因转录的蛋白质。 1. H3K4me3:通常与基因启动子区域相关联,标记着活性染色质,有利于基因的转录。H3K

Why Do Significant Gene Lists Change After Adding Additional Conditions in Differential Gene Expression Analysis?

Author: gene_x

Abstract: DESeq2 is a popular method for differential expression analysis of count data from RNA-seq experiments. It estimates fold changes and tests for differential expression using a negative binomial genera

How to extract promoters positions?

Author: gene_x

Abstract: #https://charlesjb.github.io/How_to_extract_promoters_positions/ setwd("/media/jhuang/Elements1/Data_Denise_LT_DNA_Bindung") # Load required libraries library(TxDb.Hsapiens.UCSC.hg38.knownG

How to use H3K27ac, H3K4me1, and RNA-seq to identify enhancers and their target genes?

Author: gene_x

Abstract: 1. Identify the overlapping peaks of H3K27ac and H3K4me1 using tools like BEDTools intersect or HOMER mergePeaks. H3K4me1 (histone H3 lysine 4 monomethylation) is commonly found in both active and poi

How to use H3K27me3 and H3K4me3 to identify transcription factors?

Author: gene_x

Abstract: H3K27me3 (histone H3 lysine 27 trimethylation) and H3K4me3 (histone H3 lysine 4 trimethylation) are histone marks associated with gene repression and activation, respectively. While these marks can pr

Top 15 Sequencing Companies

Author: gene_x

Abstract: As of our best knowledge, the following are some of the top sequencing companies in the genomics industry. 1. Illumina: A leading provider of DNA sequencing technologies, with platforms such as NovaS

Draw 3D PCA and calculate background genes

Author: gene_x

Abstract: [Download python3 script for generating the plot](/static/python/draw2.py "python3 script for generating the plot") [Download raw data 1 for generating the plot](/static/csv/merged_df_26PCs.csv "raw

10 NGS Categories

Author: gene_x

Abstract: 1. RNA Sequencing: RNA sequencing (RNA-Seq) is a technique used to study the transcriptome, or the complete set of RNA molecules expressed within a cell or tissue. This approach allows researchers to

交联质谱(XL-MS 或 CLMS)

Author: gene_x

Abstract: 交联质谱(XL-MS 或 CLMS)是一种强大的技术,用于研究蛋白质-蛋白质相互作用、蛋白质复合物结构和蛋白质构象变化。该方法包括使用化学交联剂将蛋白质或蛋白质之间相互作用或紧密位于的氨基酸共价连接。然后将交联蛋白质消化成肽,通过质谱分析交联肽。 交联质谱的一般工作流程如下: 1. 交联:将蛋白质或蛋白质复合物与化学交联剂处理,使其在近距离的氨基酸之间形成共价连接。交联剂的选择取决于实验要求,


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